M01 - Spinning Disk Confocal UltraView VoX
System Description
The M01 system is an inverted microscope equipped with a spinning disc (SD) unit from PelkinElmer UltraView VoX allowing fast confocal acquisition. This system can also serve as widefield microscope for brightfield and fluorescence. All components are controlled by Volocity.
The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse.
This makes it suitable e.g. for various live cell experiments or the automatic acquisition of a large amount of images (screening).
Illumination
LED: 400nm, 465nm, 565nm
Halogen Lamp 50W
Lasers for SD
405nm 100mW
488nm 100mW
561nm 100mW
Filters
Filters Vis:
Ex 400/20nm Em 460/40nm
Ex 470/40nm Em 525/50nm
Ex 562/40nm Em 624/40nm
Analyzer
Filters SD-Emission:
DIC
525/55nm
587/125nm
445/60nm; 615/70nm
485/60nm; 705/90nm
552/50nm; 640/120;
477/45nm; 575/100nm; 705/90nm
Objectives
10x/0.3 EC plan Neofluar Ph1 DICI
40x/1.3 EC plan-Neofluar Oil Ph3 DICIII
63x/1.4 plan-apochromat Oil DICIII
100x/1.46 alpha-plan-fluar Oil DICIII
Camera
SD: Hamamatsu EMCCD 9100-13 – 16µm pixel with 1.2x additional magnification
Non SD: Hamamatsu EMCCD 9100-02 – 8µm pixel
Additional devices
Motorized XY ASI stage with piezo Z.
Definite Focus
Incubation Chamber with heating and cooling.