M01 - Spinning Disk Confocal Axio Observer (inverted)

System Description

The M01 system is an inverted microscope equipped with a Yokogawa CSU X1 spinning disc (SD) allowing fast confocal acquisition. All components are controlled by the ZEN blue software.
The system is able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. There is also a ZEISS Definite Focus 2 hardware autofocus system for long-term stabilization.
This makes it suitable e.g. for various live experiments (caution: the system does not have an incubator) or the automatic acquisition of a large amount of images (screening).

 

 

 

 

Illumination

LED: 400nm, 465nm, 565nm
Halogen Lamp 50W

Laser for SD

405nm
488nm
561nm
638nm

Filters

Filters Vis:

Ex 365/12; Em LP 397; BSP 395
Ex 470/40; Em 525/50; BSP 495
Ex 550/25; Em 605/70; BSP 570


Filters SD-Emission:

450/50nm 525/50nm 562/45nm 629/62nm 490/50nm

Dichroics for Spinning Disc

405/488/568/647
475/514/647

Objectives

10x/0.3 EC plan Neofluar Ph1 DICI
32x/0.85 C-Achroplan W Korr

40x/1.3 EC plan-Neofluar Oil Ph3 DICIII
63x/1.4 plan-apochromat Oil DICIII
100x/1.46 alpha-plan-fluar Oil DICIII

Camera

SD: Hamamatsu EMCCD 9100-13 – 16µm pixel with 1.2x additional magnification

Additional devices

Motorized XY ASI stage with piezo Z.
Definite Focus

Reservation

grm3.imp.univie.ac.at