F13 - Aurora Spectral Analyzer

System Description

The Aurora from Cytek Biosciences is a novel spectral analyzer incorporating a unique combination of patent-pending innovative technologies and has the capability of measuring the entire emission spectra of the fluorescent dyes excited by multiple lasers.

It is equipped with five lasers (355nm, 405, 488, 561 and 638nm ) and can detect up to 64 fluorescence channel (and 3 scatter parameters). Samples can be acquired in tube or 96well mode.

Analysis is done via the SpectroFlo™ software which is capable of live unmixing during acquisition and extracting autofluorescence.

The system is situated in the Busslinger lab.

 

 

Laser 1

355 nm UV Laser, 20mW

Detection

16 channels uneven spaced bandwidth from 420nm-829nm

 

Laser 2

405 nm Violet Laser, 100mW

Detector module

16 channels uneven spaced bandwidth from 420nm-829nm

 

Laser 3

488 nm Blue Laser, 50mW

Detector module

14 channels uneven spaced bandwidth from 498-829nm

 

 

Laser 4

561 nm Yellow-Green Laser, 50mW

Detector module

10 channels uneven spaced bandwidth from 567-829 nm

 

 

Laser 5

638 nm Red Laser, 80mW

Detector module

8 channels uneven spaced bandwidth from 652-829nm