F11 - Sony SH800 Cell Sorter

System Description

The SONY SH800 is a three laser (405nm, 488 nm and 561 nm) sorter which can be operated by users themselves after having received training. It is capable of simultaneous detection of 2 scatter and 6 fluorescent parameters and allows bulk sorts of up to two populations as well as (index-)sorting in various plate types (e.g. 6, 24, 48, 96, 384 wells). It is mainly suited to sort GFP and mCherry/RFP labeled cells.

 

 

Laser

Colinear Excitation:

405nm Violet Laser, 20mW

488nm Blue Laser, 20mW

561nm Yellow- Green Laser, 20mW

Detection

450/50 nm - FL1, BFP, DAPI, Hoechst, e-fluor405, BV421

525/50 nm - FL2, GFP, FITC, AF 488, YFP, mCitrine,

600/60 nm - FL3, PE, PE-Texas Red, PI, DsRed, tdTomato, mCherry,

665/30 nm - FL4, 7-AAD, PE-Cy5, PerCP, mPlum

720/60 nm - FL5, PE-Cy5.5, PerCP-Cy5.5,

785/60 nm - FL6, PE-Cy7