F07 - FACS LSR Fortessa

System Description

The FACS LSR Fortessa is a 3 laser flow cytometer with 14 fluorescence detectors controlled by FACSDiva software. It is especially suited for multi-colour experiments.

Laser 1

405 nm 100 mW Violet laser

Detection

450/50 nm - Alexafluor 405, Cascade Blue, DAPI, Hoechst, Pacific Blue
525/50 nm - Alexafluor 430, AmCyan, Pacific Orange
610/20 nm - Qdot 605
670/30 nm - Qdot 655
710/50 nm - Qdot 705
780/60 nm - Qdot 800

Laser 2

488 nm Blue laser (100 mW)

Detection

488/10 nm - SSC

530/30 nm - FITC, GFP, YFP

695/40 nm - PerCP-Cy5.5, Cychrome

Laser 3

640 nm Red laser (40 mW)

Detection

670/30 nm - APC, Alexa Fluor 647
730/45 nm - Alexa Fluor 700
780/60 nm - APC-CY7, APC-H7

Laser 4

561 nm Yellow-green laser

Detection

582/15 PE, DsRed, dTomato

610/20 PE-Texas Red, PI, Living Colors, m-Cherry, m-Raspberry

670/30 PE-Cy5

710/50 PE-Alexa Fluor, PE-Cy5-5

780/60 PE-Cy7