F05 - FACS Aria IIu

System Description

The FACS Aria IIu is a 3 laser sorter with 10 fluorescence detectors controlled by FACSDiva software and operated exclusively by BioOptics staff members. It allows bulk sorts of up to 4 populations simultaneously. Temperature control for sample input and sort output (4oC to room temperature) is possible. The nozzle sizes 70μm - 85μm - 100μm - 130μm  are availabe.

 

Fluorochromes in bold letters are listed in the configurations of the flow cytometer.

Laser 1

405 nm Violet Laser, 50mW

Detection

450/40 nm - AF 405, BFP, BV 421, Cascade Blue, DAPI, Hoechst 33342, Pacific Blue
530/30 nm - AF 430, AmCyan, Ametrine, BD Horizon V500, BV 510, QDot 565
785/60 nm - BV 786, QDot 800, (BV570 in Configuration)

Laser 2

488 nm Blue Laser, Coherent Sapphire 20mW

Detection

488/10 nm - SSC
530/30 nm - AF 488, FITC, GFP, Venus, YFP
575/26 nm - PE
610/20 nm - PE-Texas Red, PI
675/20 nm - 7-AAD, CyChrome, PE-Cy5, PerCP, PerCP-Cy5.5
780/60 nm - PE-Cy7

Laser 3

633 nm Red Laser, HeNe 17mW

Detection

675/20 nm - AF 647, AF 660, APC
780/60 nm - APC-Cy7, APC-H7

 

Reservation

facs@imp.ac.at