F05 - FACS Aria III
System Description
The FACS Aria III is a 4 laser sorter with 16 fluorescence detectors controlled by FACSDiva software and operated exclusively by BioOptics staff members. It allows bulk sorts of up to 4 populations simultaneously as well as single cell sorting. It is therefore best suited for sorting onto slides and into multi-well plates. Temperature control for sample in and out is possible. Both high and low speed sortings can be performed.
Laser 1
375 nm Near UV Laser, 7mw (output laser head)
Detection
780/60 nm - Qdots 800
710/50 nm - Qdots 705
670/30 nm - Qdots 655
605/12 nm - Qdots 605
530/23 nm - Qdots 525, Qdots 545, Pacific Orange, Cascade Yellow
450/40 nm - DAPI, Alexa Fluor 350, Hoechst 33258, Calceine Violet, BD Horizon 450, Pacific Blue, Cascade Blue, Marina Blue, DyeCycle Violet
Laser 2
488 nm Coherent Sapphire Solid State 13mW
Detection
488/10 nm - Side scatter (SSC)
530/30 nm - FITC, GFP, YFP, Alexa 488
695/40 nm - PerCP-Cy5.5
Laser 3
561 nm Laser, 50 mW (output laser head)
Detection
780/60nm - PE-Cy7
710/50nm - PE-Cy5.5
670/14nm - PE-Cy5, PerCP, mPlum, Nile Red,
610/20nm - PI, Texas-Red, mCherry, mRaspberry, PE-Texas Red, Alexa Fluor 568, PE Alexa Fluor 610, SNARF1 (pH9)
582/15nm - PE, CF555, Cy3.5, Cy3B, DsRed, dTomato, Mito Tracker Red, mOrange, Rhodamine Red, SNARF1 (pH6), TMRho
Laser 4
633 nm 11mw
Detection
660/20 nm APC, Alexa Fluor 647
780/60 nm APC Cy5, APC Cy7