F01 - FACS LSR Fortessa

System Description

The FACS LSR Fortessa is a 4 laser flow cytometer with 16 fluorescence detectors controlled by FACSDiva software. It is especially suited for multi-colour experiments.

The F01 FACS LSR II Fortessa is additionally equipped with a HTS High Throughput Sampler for 96 well plates.

 

Fluorochromes in bold letters are listed in the configurations of the flow cytometer.

 

20160421

Laser 1

405 nm Violet Laser, Coherent Obis 100 mW

Detection

424/46 nm - AF 405, BFP, BV 421, Cascade Blue, DAPI, Hoechst 33342, Pacific Blue
525/50 nm - AF 430, AmCyan, Ametrine, BD Horizon V500, BV 510, QDot 565
600/32 nm - BV 570, BV 605, Pacific Orange, QDot 605
670/30 nm - BV 650, QDot 655
710/50 nm - BV 711, QDot 705
800/50 nm - BV 786, QDot 800

Laser 2

488 nm Blue Laser, Coherent Sapphire 100 mW

Detection

488/10 nm - SSC
530/30 nm - AF 488, FITC, GFP, Venus, YFP
695/40 nm - CyChrome, PerCP, PerCP-Cy5.5

Laser 3

561 nm Yellow Green Laser, Coherent Sapphire 100mW

Detection

582/15 nm - DsRed, PE, tdTomato
610/20 nm - Living Colors, mCherry, mRaspberry, PE-Texas Red, PI
670/30 nm - 7-AAD, PE-Cy5
710/50 nm - PE-Cy5.5, PE-AF 700
780/60 nm - PE-Cy7

 

 

Laser 4

640 nm Red laser, Coherent Cube (635-640) 40 mW

Detection

670/30 nm - AF 647, AF 660, APC
730/45 nm - AF 700
780/60 nm - APC-Cy7, APC-H7