F01 - FACS Calibur

System Description

The FACS Calibur is a 2 laser flow cytometer with 4 fluorescence detectors controlled by Cellquest Pro software. It is especially suited to analyse cells containing fluorescently tagged proteins as well as 4 color experiments.

Laser 1

488 nm Air-cooled argon-ion, 15 mW

Detection

488/10 nm - SSC
530 nm - FITC, GFP, YFP, Alexa 488
585 nm - PE,PI ds-Red
>650 nm - PerCP-Cy5.5,  PerCP, Pe-Cy5.5

Laser 2

635 nm red Diode

Detection

661 nm - APC, Alexa 647