
M19 - Pannoramic SCAN 150
System Description

The M19 system is an automated widefield microscope equipped with transmitted and fluorescent light source. It can be used to scan up to 150 slides in both transmitted and fluorescence. The advantages of this system are the constant illumination and white balance as well as the elimination of the operator induced error. The system can acquire Z-stacks and deliver maximum intensity projection (MIP) important for 3D structures.
As a result you get images of the whole sample (and not only of the "preferentially" selected area) that can be subjected to automated image analysis. The system can run on its own, no attendance needed.
Slides for the automatic scanner have to be prepared in a specific way. Please ask us for directions or refer to Protocols. This system is exclusively operated by the BioOptics facility staff. It helps to automate your experiments and fits into the chain of - automated staining - imaging - image analysis.
Illumination
Sola Spectra – Solid state light engine
Halogen Lamp 50W
Filters
Ex 365/12nm Em 397/LP
Ex 470/40nm Em 525/50nm
Ex 550/25nm Em 605/70nm
Ex 620/60nm Em 700/70nm
Objectives
20/0.8 plan-apochromat
Camera
Hitachi HVF22 – transmitted light – pixel size on the image - 0,23387µm
Axiocam MRm – fluorescence – pixel size on the image – 0,3225
Reservation
Please contact BioOptics Staff